Journal: Scientific Reports

Article Title: Six Sigma model with implement SAPF to enhance power quality and product quality at induction heat treatment process

doi: 10.1038/s41598-025-03787-x

Figure Lengend Snippet: A detailed connection diagram accuSine PCS+, will be connected to the existing MSB switchboard.

Article Snippet: Provide Active Harmonic Filter with Characteristics Active Harmonic Filter AccuSine PCS + 300A, including Encloser IP31, Main incoming MCCB 630A, AccuSine harmonic filter integrated 300A, Ventilation Fan, USB communication port, Ethernet Cable 5m, Display: HMI 5.7’’ touch screen with detail information about current, voltage, THDi, THDv, event logs, and Manufacture: Schneider Electric (Shown in Fig. ).

Techniques:

Journal: Science translational medicine

Article Title: Graft-derived extracellular vesicles transported across subcapsular sinus macrophages elicit B cell alloimmunity after transplantation

doi: 10.1126/scitranslmed.abb0122

Figure Lengend Snippet: (A and B) Representative flow cytometry analysis (A) and quantification (B) in pLNs of binding of syngeneic and allogeneic EVs (CM-DiI–labeled, footpad-injected) by CMAC-labeled, intravenously injected alloreactive B cells in presence or absence of SCS macrophages. SCS macrophages were depleted by administration of low-dose clodronate liposomes via footpad injection. (C and D) Representative flow cytometry analysis (C) and quantification (D) in pLNs of CD86 expression by CMAC-labeled, intravenously injected alloreactive B cells in response to syngeneic or allogeneic EVs (CM-DiI–labeled, footpad-injected), in presence or absence of SCS macrophages. (E and F) Mobilization to the B cell–T cell border in pLNs of CFSE-labeled, intravenously injected alloreactive B cells after footpad injection of syngeneic or allogeneic EVs in presence of SCS macrophages or after depletion. B cell migration was assessed 20 hours after EV injection. Images representative of eight LNs per condition. (G) Detection by flow cytometry of DSAs in serum of mice before and on consecutive days after footpad injection of syngeneic or allogeneic EVs. Mice were left untreated or injected once with control or low-dose clodronate liposomes (footpad and intravenous) 5 days before EV injection. Dots represent individual mice. In (B), (D), and (F), each dot represents pLNs pooled from one mouse. In (A) to (G), an equivalent of 55 × 108 EVs (85 ± 39 nm in size) or syngeneic EVs (109 ± 49 nm in size) in 30 μl of PBS was injected per footpad. Results were analyzed by one-way ANOVA, followed by Tukey-Kramer multiple comparison test. Error bars denote means ± SD. **P < 0.01 and ***P < 0.001.

Article Snippet: The following sets of filters were used: (i) 420- to 460-nm (CMAC and secondary harmonic), 495- to 540-nm (CFSE, AF488, YFP, and Fluo-4 AM), 573- to 647-nm (CM-Dil), and 660- to 760-nm (AF647) detection filters (Olympus) and a 690-nm IR cutoff filter (Olympus), in the Olympus FV1000 microscope; (ii) 460- to 500-nm (CMAC and secondary harmonic), 500- to 550-nm (CFSE, AF488, YFP, and Fluo-4 AM), 570- to 620-nm (CM-Dil), and 665- to 760-nm (AF647) detection filters (Nikon) and a 750-nm IR cutoff filter (Nikon), in the A1R MP Nikon multiphoton system; or (iii) 555-nm primary dichroic mirror with 495- and 640-nm secondary dichroic mirrors with band-pass filters 460/60 nm (CellTrace Violet), 520/35 nm (AF488), 575/19 nm (CM-Dil, tagRFP), and 655/15 nm (CellTrace Far Red) in the Leica multiphoton system.

Techniques: Flow Cytometry, Binding Assay, Labeling, Injection, Expressing, Migration

Journal: Science translational medicine

Article Title: Graft-derived extracellular vesicles transported across subcapsular sinus macrophages elicit B cell alloimmunity after transplantation

doi: 10.1126/scitranslmed.abb0122

Figure Lengend Snippet: (A) Image of a pLN showing tracks of alloreactive B cells contacting SCS macrophages with allogeneic EVs. (B) Instant velocities of individual B cell tracks from (A). (C) Mean velocities and arrest coefficients of alloreactive B cells contacting SCS macrophages with allogeneic EVs. (D) Cell tracks of B cells in proximity to SCS macrophages with allogeneic EVs. (E) Parameter comparison by two-photon microscopy between alloreactive and control B cells in pLNs, after footpad injection of allogeneic EVs. (F) Sequence of calcium flux in an alloreactive B cells contacting SCS macrophages with allogeneic EVs in pLNs. (G) Mobilization of alloreactive B cells to the B cell–T cell border of pLNs, after footpad injection of allogeneic EVs. X200. (H) Sequence of passage of allogeneic EVs to alloreactive B cells in pLNs (arrow, inset). (I and J) Flow cytometry analysis (I) and quantification (J) in pLNs of binding of CM-DiI-EVs to intravenously (i.v.) injected alloreactive (CMAC) or control (CFSE) B cells or endogenous B cells. (K) Flow cytometry analysis in pLNs of CD86 by intravenously injected alloreactive B cells after footpad injection of allogeneic or syngeneic CM-DiI-EVs. In (A) to (K), an equivalent of 55 × 108 allogeneic EVs (85 ± 39 nm in size) or syngeneic EVs (109 ± 49 nm in size) in 30 μl of PBS was injected per footpad. (A to K) Results are representative of three to nine pLNs per condition. In (J) and (K), each dot represents a pLN. Results analyzed by two-tailed Student’s t test and one-way ANOVA, followed by Tukey-Kramer multiple comparison test. Error bars denote means ± SD. **P < 0.01, ***P < 0.001, and ****P < 0.0001.

Article Snippet: The following sets of filters were used: (i) 420- to 460-nm (CMAC and secondary harmonic), 495- to 540-nm (CFSE, AF488, YFP, and Fluo-4 AM), 573- to 647-nm (CM-Dil), and 660- to 760-nm (AF647) detection filters (Olympus) and a 690-nm IR cutoff filter (Olympus), in the Olympus FV1000 microscope; (ii) 460- to 500-nm (CMAC and secondary harmonic), 500- to 550-nm (CFSE, AF488, YFP, and Fluo-4 AM), 570- to 620-nm (CM-Dil), and 665- to 760-nm (AF647) detection filters (Nikon) and a 750-nm IR cutoff filter (Nikon), in the A1R MP Nikon multiphoton system; or (iii) 555-nm primary dichroic mirror with 495- and 640-nm secondary dichroic mirrors with band-pass filters 460/60 nm (CellTrace Violet), 520/35 nm (AF488), 575/19 nm (CM-Dil, tagRFP), and 655/15 nm (CellTrace Far Red) in the Leica multiphoton system.

Techniques: Microscopy, Injection, Sequencing, Flow Cytometry, Binding Assay, Two Tailed Test